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Functional analysis of HIV -1 reverse transcriptase motif C: site directed mutagenesis and metal cation interaction




, : Functional analysis of HIV -1 reverse transcriptase motif C: site directed mutagenesis and metal cation interaction. Journal of Molecular Evolution 47(1): 73-80

The RNA replicase activity induced by manganese on HIV-1 reverse transcriptase (RT), its simultaneous inactivation in a mutant that almost abolishes DNA polymerase activity, and the change in activating cation preference for DNA polymerase activity in the same mutant all support the proposal that: (1) the Asp 186 residue, essential for catalysis, is part of the metal binding site of the HIV-1 RT; (2) the active site containing this residue is responsible for both the natural DNA polymerase ac...

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