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A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique


A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique



Plant Disease Reporter 57(12): 1025-1028



Five techniques for preparing inocula of Meloidogyne incognita and M. hapla were compared. These were: (i) light brown intact egg masses, (ii) egg masses fragmented with a Ten-Broek tissue homogenizer, (iii) eggs collected by the Coolen & D'Herde procedure, (iv) larvae collected by incubating infected roots in a mist chamber for 8 to 12 hours and (v) eggs collected by dissolving gelatinous matrices in sodium hypochlorite solution at concentrations of 0.53%, 1.05% or 2.62% at 21 deg C.

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