Callus formation from mesophyll protoplasts of Pisum sativum

Constabel, F.; Kirkpatrick, J.W.; Gamborg, O.L.

Canadian Journal of Botany 51(11): 2105-2106


ISSN/ISBN: 0008-4026
DOI: 10.1139/b73-272
Accession: 000034402

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Protoplasts isolated from mesophyll of the variety Century and cultured in 0.2 ml droplets of B5 medium with 1.0 mg/l 2,4-D or alpha -naphthylacetic acid and 2.0 mg/l kinetin regenerated cell walls within 2-3 days and the resulting cells began to divide and form callus tissue after 19 days. Protoplast survival depended on keeping leaves in the dark for at least 30 h before use.