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Cell culture adaptation and propagation of a reovirus-like agent in calf diarrhea from a field outbreak in Nebraska



Cell culture adaptation and propagation of a reovirus-like agent in calf diarrhea from a field outbreak in Nebraska



Archiv fur die Gesamte Virusforschung 37(1): 114-130



The Nebraska calf diarrhea viral agent [see V.B. 41, abstr. 5709] was successfully adapted and propagated in several cell culture systems. Criteria of propagation were: production of a cytopathic effect, specific fluorescence, interference with cytopathogenicity of another virus, infection of neonatal calves, and identification of the agent by electron microscopy or immuno-electron microscopy. These techniques proved to be valuable diagnostic aids for detecting the disease in calves. Morphologically, and in resistance to lipid solvents and fluorocarbon, the agent resembled the reoviruses. However, it was serologically unrelated to reoviruses, so a 'reovirus-like' classification for this virus was suggested.

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Accession: 000036166

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PMID: 4623479


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