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Detection of a latent infectious agent that protects against infection by chrysanthemum chlorotic mottle viroid

, : Detection of a latent infectious agent that protects against infection by chrysanthemum chlorotic mottle viroid. Phytopathology 65(9): 1000-1003

A latent infectious agent (ChCMV-NS) that protects against ChCMV was found in cvs. of Chrysanthemum morifolium; it produced no recognizable symptoms but is detectable by its complete protection against the severe str. of ChCMV. At least 8 days of incubation are required after inoculation of ChCMV-NS for complete protection against ChCMV. Opt. detection of ChCMV-NS occurred at 21 deg C and the opt. for detection of ChCMV from crude extracts of source plants was 21-24 deg.

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Related references

Horst R.K.; Kawamoto S.O., 1975: Some properties of a latent infectious agent which protects against chrysanthemum chlorotic mottle viroid. Proceedings of the American Phytopathological Society 2: 98

Jeon, S.Min.; Naing, A.Htay.; Kim, H-Hoon.; Chung, M.Young.; Lim, K.Byung.; Kim, C.Kil., 2015: Elimination of chrysanthemum stunt viroid and chrysanthemum chlorotic mottle viroid from infected chrysanthemum by cryopreservation. Chrysanthemum morifolium 'Borami' and 'Secret Pink' showing symptoms of stunt disease caused by chrysanthemum stunt viroid (CSVd) and 'Yellow Cap' showing chlorotic mottle disease caused by chrysanthemum chlorotic mot...

Cho, W.Kyong.; Jo, Y.; Jo, K-Min.; Kim, K-Hyung., 2013: A current overview of two viroids that infect chrysanthemums: Chrysanthemum stunt viroid and Chrysanthemum chlorotic mottle viroid. The chrysanthemum (Dendranthema X grandiflorum) belongs to the family Asteraceae and it is one of the most popular flowers in the world. Viroids are the smallest known plant pathogens. They consist of a circular, single-stranded RNA, which does no...

Park, J.; Jung, Y.; Kil, E-Joon.; Kim, J.; Thi Tran, D.; Choi, S-Kook.; Yoon, J-Yeon.; Cho, W.Kyong.; Lee, S., 2014: Loop-mediated isothermal amplification for the rapid detection of Chrysanthemum chlorotic mottle viroid (CChMVd). Loop-mediated isothermal amplification (LAMP) is an established nucleic acid amplification method offering rapid, sensitive, and convenient diagnosis of infectious diseases. Chrysanthemum chlorotic mottle viroid (CChMVd) causes one of the most ser...

Macquaire, G.; Monsion, M.; Bachelier, J.C.; Faydi, C.; Dunez, J., 1981: The slab gel electrophoretic assay for detection and investigation of chrysanthemum chlorotic mottle viroid (CChMV) in infected plants. Electrophoretic assay with slab gels stained with ethidium bromide was highly sensitive, reliable and versatile in detecting the viroid causing disease in chrysanthemum.

Flores, R.; Pena, M. de la; Navarro, B., 2003: Chrysanthemum chlorotic mottle viroid. This paper provides details of the host range, transmission, geographical distribution, epidemiology, detection, control, taxonomic position and nucleotide sequence of chrysanthemum chlorotic mottle viroid.

Yamamoto, H.; Sano, T., 2005: Occurrence of Chrysanthemum chlorotic mottle viroid in Japan. Chrysanthemum chlorotic mottle viroid (CChMVd) was detected in Akita Prefecture, Japan, from chrysanthemums (Dendranthema grandiflorum) with distinct yellow leaf mottling and necrosis. The four clones are 398-399 nucleotides long and are thought t...

Romaine, C.P.; Horst, R.K., 1975: Suggested viroid etiology for chrysanthemum chlorotic mottle disease. At pH 5.5 - 6.5 the agent of this disease (ChCMV) is extremely unstable in crude leaf extracts. Grinding infected tissue in alkaline buffer (pH 7.5 - 9.5) significantly increased stability and infectivity. Homogenization of tissue in 0.2 M borate...

Li, M.J.; Yang, X.C.; Tien, P., 1987: Identification of Chinese chrysanthemum chlorotic mottle viroid (C-ChMV). A small molecular nucleic acid was isolated form naturally infected chrysanthemum plants cv. Guangbai showing chlorotic mottle symptoms in Guangzhou province. Chrysanthemum chlorotic mottle viroid was identified on the basis of host tests and a fr...

Kawamoto, S.; Horst, R.; Wong, S., 1985: Solubility of chrysanthemum chlorotic mottle viroid in LiCl solutions. Acta Horticulturae 164: 333-340