Differentiation between periodic and subperiodic Brugia malayi and Brugia pahangi on the basis of microfilarial sheath-casting in vitro

Sivanandam, S.; Dondero, T.J.

Annals of Tropical Medicine and Parasitology 66(4): 487-496


ISSN/ISBN: 0003-4983
PMID: 4656450
DOI: 10.1080/00034983.1972.11686851
Accession: 000061477

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Under certain conditions, periodic and sub(-)periodic Brugia malayi and B. pahangi were distinguishable from one another on the basis of microfilarial sheath-casting in vitro. When fresh thick blood films were allowed to dry normally, away from air-conditioning, most periodic B. malayi exsheathed while almost none of the sub(-)periodic B. malayi or B. pahangi did so. Under the most favourable conditions (incubation of blood film for 2 hours in a refrigerated, moist chamber), the B. pahangi exsheathing rate was consistently enhanced to more than 25% while the sub(-)periodic B. malayi rate was well under 10%. Accelerated drying conditions and the presence of anticoagulants reduced or suppressed sheath-casting by all 3 parasites. [AS].