Effect of sodium linoleate infusion on plasma free fatty acids, glucose, insulin, and ketones in unanesthetized dogs

Crespin, S.R.; Greenough, W.B.; Steinberg, D.

Diabetes 21(12): 1179-1184


ISSN/ISBN: 0012-1797
PMID: 4636212
DOI: 10.2337/diab.21.12.1179
Accession: 000075072

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By using a continuous-flow centrifuge to separate carotid artery blood into cells and plasma, sodium linoleate was infused into the jugular vein of 5 conscious dogs at a mean rate of 41.9 mu -equiv/kg min for 90 min without adverse side effects. Linoleate infusion quickly increased plasma fatty acid (FFA) values to about 1.50 mu -equiv/ml and was accompanied by a rapid four- to six-fold increase in plasma immunoreactive insulin (IRI) which was sustained throughout the infusion. After 30 min of infusion, plasma glucose began to fall, ultimately declining 35% from control values. Plasma ketone concentrations rose to twice their control values during linoleate infusion but were always less than 20% of those shown to be insulinogenic in dogs. When the infusion ended, plasma FFA, IRI and ketones fell quickly to control values, while plasma glucose rose towards normal.The half-life of plasma linoleate, estimated from the infusion rate and the increment in plasma FFA during steady-state infusion, was 0.76 min. This result, based on net flux, validates estimates based on tracer experiments. Stimulation of insulin secretion by high concentrations of FFA could play a role in modulating lipolytic responses and changes in glucose metabolism accompanying such responses.