Histamine-stimulated hydrogen ion secretion by in vitro piglet gastric mucosa
Forte, J.G.; Forte, T.M.; Machen, T.E.
Journal of Physiology 244(1): 15-31
ISSN/ISBN: 0022-3751 PMID: 1123738 DOI: 10.1113/jphysiol.1975.sp010782
The gastric mucosa of piglets up to 5 days old was removed with minimal trauma from the outer muscle layers of the stomach by injecting warm, oxygenated Ringer's solution between the layers and peeling off the resulting 'blister'. At rest in suitably buffered Ringer's solution the mucosa remained stable for several hours, secreted no acid and had an electrical potential difference (p.d.) of -30 mV. Acid secretion commenced 3 minutes after the addition of histamine to the solution, while p.d. increased and resistance decreased. Maximally stimulating doses of histamine (10-8M) caused hydrogen ion secretion to increase up to 15 mu equiv./cm2 hr.