In vivo and in vitro effects of synthetic luteinizing hormone-releasing hormone (LH-RH) upon the secretion of luteinizing hormone (LH) and follicle stimulating hormone (FSH) in intact and castrated, fed and starved adult male rats

Root, A.W.; Duckett, G.E.

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 144(1): 30-33


ISSN/ISBN: 0037-9727
PMID: 4589214
DOI: 10.3181/00379727-144-37520
Accession: 000113910

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The effects of a synthetic hypothalamic luteinizing hormone-releasing hormone (LH-RH) were studied in vivo and in vitro. Adult males rats in standardized conditions were used, half were castrated and then randomly assigned to "fed" or "starved" groups. The latter received only water for 168 h. Half of each group were then killed and the anterior pituitaries were incubated with or without LH-RH, while the others were anaesthetized and 0.1 mu g/100 g bodyweight of LH-RH was injected and blood was sampled for analysis after 15 or 45 min. The LH-RH failed to affect the serum concentrations of follicle-stimulating hormone (FSH), but LH concentrations increased in both intact and castrated, fed and starved rats. In vitro, the basal and LH-RH-induced secretion of LH and FSH from all 4 groups was similar. It was concluded that it is the hypothalamus which is sensitive to inanition.