EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Studies on human secretory IgA. II. Comparative studies on a fragment of secretory component derived from secretory IgA and fragments obtained by enzymatic digestion of free secretory component



Studies on human secretory IgA. II. Comparative studies on a fragment of secretory component derived from secretory IgA and fragments obtained by enzymatic digestion of free secretory component



Immunochemistry 10(2): 73-80



Reduced and alkylated secretory IgA (SIgA) from human milk released a fragment resembling the secretory component (SC) but having a smaller mol. wt. (34 000-45 000), while also lacking certain antigenic determinants of SC. Reductive cleavage did not affect the mol. wt. or antigenic specificity of free SC (FSC) present in milk. However, enzymatic digestion of FSC by various proteases released fragments which closely resembled the above described fragment from bound SC with respect to mol. wt., antigenic specificity and electrophoretic mobility. Trypsin, chymotrypsin and subtilisin were all found to be very effective in producing these changes, whereas pepsin was less efficient. Compared to FSC, the SC bound to SIgA was resistant to the attack by chymotrypsin. All the SC fragments studied, whether obtained by proteolysis of FSC or by reduction of whole SIgA, showed marked multi-banded electrophoretic polymorphism.

(PDF emailed within 0-6 h: $19.90)

Accession: 000204753

Download citation: RISBibTeXText

PMID: 4125505

DOI: 10.1016/0019-2791(73)90233-4



Related references

Characterization of epitopes of human secretory component on free secretory component secretory immunoglobulin a and membrane associated secretory component. Journal of Immunology 133(4): 2116-2125, 1984

Characterization of epitopes of human secretory component on free secretory component, secretory IgA, and membrane-associated secretory component. Journal of Immunology 133(4): 2116-2125, 1984

Studies on protein components particularly secretory immunoglobulin a and free secretory component in bronchoalveolar lavage fluids. Shikoku Acta Medica 40(4): 684-694, 1984

Human secretory component part 2 physicochemical characterization of free secretory component purified from colostrum. Scandinavian Journal of Immunology 3(6): 707-716, 1974

Comparison of the cyanogen bromide fragments of canine secretory immuno globulin a and free secretory component. Federation Proceedings 35(3): 272, 1976

Human secretory immunoglobulins. III. Immunochemical and physio-chemical studies of secretory IgA and free secretory piece. Acta path, microbiol. scand., B, 79: 2, 165-188, 1971

Human secretory immunoglobulins. 3. Immunochemical and physicochemical studies of secretory IgA and free secretory piece. Acta Pathologica et Microbiologica Scandinavica. Section B: Microbiology and Immunology 79(2): 165-188, 1971

Human secretory component. II. Physicochemical characterization of free secretory component purified from colostrum. Scandinavian Journal of Immunology 3(6): 707-716, 1974

Human secretory component part 1 purification of free secretory component from colostrum. Scandinavian Journal of Immunology 3(5): 579-588, 1974

Studies on human secretory IgA comparative studies of the IgA-bound secretory piece and the free secretory piece protein. Immunochemistry 8(9): 785-800, 1971

Human secretory component. I. Purification of free secretory component from colostrum. Scandinavian Journal of Immunology 3(5): 579-588, 1974

Human secretory component. IV. Aggregation and fragmentation of free secretory component. Immunochemistry 12(11): 877-881, 1975

Esterase activity associated with secretory IgA and free secretory component preparations from human milk. Biochimica et Biophysica Acta 317(2): 517-523, 1973

Esterase activity associated with secretory IgA and free secretory component preparations from human milk. Biochimica et Biophysica Acta 317(2): 517-523, 2010

Purification of free secretory component and secretory immuno globulin a from human milk. Federation Proceedingspart 1: 968, 1973