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Studies on the assembly of Newcastle disease virus: incorporation of structural proteins into virus particles



Studies on the assembly of Newcastle disease virus: incorporation of structural proteins into virus particles



Journal of General Virology 12(Part 3): 239-247



When protein synthesis was inhibited by addition of puromycin, cycloheximide or fluoro-phenylalanine at any time during the course of infection, subsequent virus production was soon inhibited, even when added to the cultures as late as 10 hours after infection, when large amounts of virus precursor proteins were present within the cell. The kinetics and efficiency of incorporation of radioactive amino acid into virus particles were examined by the pulse-labelling technique. A 30 min. labelling period at the 3rd or 6th hour of infection resulted in the release of highly radioactive virus during the period of 11/2 hours immediately after the pulse. However, when pulse-labelling was performed at the 9th hour, the maximally labelled virus was found in the yield obtained 3 hours after the pulse, and the specific radioactivity of virus was less than 1% of the virus harvested from cultures labelled earlier. On the basis of these findings, possible mechanics of virus assembly are discussed.

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Accession: 000206133

Download citation: RISBibTeXText

PMID: 5165852

DOI: 10.1099/0022-1317-12-3-239


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