Synthesis of nitrogen compounds from non-protein nitrogen sources in the liver of sheep

Kosharov, A.N.; Kurilov, P.N.

Doklady Vsesoyuznoi Ordena Lenina Akademii Sel' skokhozyaistvennykh Nauk 8: 22-24


Accession: 000213931

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The livers of 10 sheep 1.5 to 2.0 years old of body weight 25 to 30 kg and fed on hay and concentrates were perfused with solutions without or containing 15N-labelled diammonium phosphate or ammonium chloride. Samples of liver and blood perfusate were taken before and at intervals during infusion for estimation of radioactivity, amino acids and proteins. Compared with the controls each of the labelled ammonium salts increased the concentrations of glutamic acid, arginine, aspartic acid, valine, leucine, isoleucine, threonine, glycine and tyrosine and the total free amino acids in plasma. During perfusion the concentrations in plasma of serine, alanine, methionine and phenylalanine fell in both the control and test groups. 15N was incorporated into essential and non-essential amino acids. In blood the highest radioactivity was found in arginine, aspartic acid and glutamic acid; none in histidine and glycine. In the liver 15N was incorporated mostly into lysine, aspartic and glutamic acids and alanine. 15N was also detected in polypeptides and blood protein fractions (albumins, alpha 1-, alpha 2-, beta - and gamma -globulins) and beta -lipoproteins as well as in purine and pyrimidine nucleotides.