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The effect of protein quality and ascorbic acid deficiency on stimulation of hepatic microsomal enzymes in guinea pigs



The effect of protein quality and ascorbic acid deficiency on stimulation of hepatic microsomal enzymes in guinea pigs



Toxicology and Applied Pharmacology 24(4): 603-611



Studies were made on 5 groups each of 4 female guineapigs. Three groups were given diets based on casein either with adequate ascorbic acid or with DDT and lindane but without ascorbic acid, or (controls) with ascorbic acid, DDT and lindane, the fourth group was given a diet based on soya protein in place of casein with ascorbic acid, DDT and lindane and the fifth group a diet based on gelatin with ascorbic acid, DDT and lindane. Daily food intake and weight changes were recorded. D-Glucaric acid and lindane and its metabolites were estimated in 24-h urine samples and on day 15 the animals were killed and their livers were removed for estimation of cytochrome P-450 and the activity of various enzymes; other studies of enzyme activity included those responsible for O-demethylation of p-nitroanisole, oxidative hydrolysis of O-ethyl O-(4-nitrophenyl) phenylphosphothionate (EPN), azo group reduction in methyl orange, epoxidation of aldrin to dieldrin and glucuronidation of p-nitrophenol.After 1 week urinary excretion of glucaric acid was significantly greater in the group given soya protein than in all other groups; neither the deprived guineapigs nor those given gelatin excreted significantly more glucaric acid than the controls at any time during the study. Protein inadequacy supported the significant stimulation of O-demethylase but impaired the oxidative hydrolysis of EPN induced by the pesticides. Soya protein supported significantly higher increases in cytochrome P-450 and glucuronyl transferase whereas gelatin did not. Ascorbic acid deficiency depressed cytochrome P-450 and the O-demethylation activity induced by the pesticides and significantly increased glucuronyl transferase activity. Soya protein but not gelatin enhanced the excretion of lindane and its metabolites over the controls whereas ascorbic acid deficiency impaired it. It is concluded that poor quality protein can support the metabolic responses to DDT and lindane whereas ascorbic acid deprivation impairs most of the observed responses.

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Accession: 000227526

Download citation: RISBibTeXText

PMID: 4123385

DOI: 10.1016/0041-008x(73)90222-6


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