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Tobacco hornworm: marking the spermatophore with water-soluble stains



Tobacco hornworm: marking the spermatophore with water-soluble stains



Journal of Economic Entomology 66(3): 719-721



Certain water-soluble vital stains administered to adult males of Manduca sexta (Joh.) by feeding or by injection into the haemocoel in the laboratory in North Carolina were found to be effective in marking spermatophores. Injection was the more efficient technique and did not damage the moths. Out of more than 60 stains evaluated, only rhodamine B, methylene blue chloride, pararosaniline acetate and pararosaniline hydrochloride stained the spermatophores.

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Accession: 000251116

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DOI: 10.1093/jee/66.3.719


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