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A further simplified single immunodiffusion drop test for the detection of lily symptomless virus in Lilium species


, : A further simplified single immunodiffusion drop test for the detection of lily symptomless virus in Lilium species. Acta Horticulturae (59): 29-36

A further simplification of the single immunodiffusion drop test was achieved by replacing agar, incorporated in the pyrrolidine-extraction medium as a coagulant, by the starch derivative 'Saroma'. In a single experiment the minimum amount of the virus detectable was c. 1/8 of that by the Ouchterlony double diffusion test.


Accession: 000271079

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Related references

Slogteren, D.H.M.Van, 1976: A single immunodiffusion drop test for the detection of lily symptomless virus. In the test, developed for detection of the virus in lilies, its presence is demonstrated by intra-gel precipitation in drops of an antiserum agar mixture which are contiguous with droplets of pyrrolidine treated leaf extracts covered with paraffi...

Slogteren, D.-Van; Beijersbergen, J.1; Bunt, M.1; Hulst, C.-Van-Dero, 1980: Detection of lily symptomless virus in leaves and bulb scales of lily plants with the immunodiffusion drop test and with ELISA enzyme-linked immunosorbent assay. Acta Horticulturae: (110) 91-98

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