A method for determining amino acid concentrations and specific activities of amino acids and some other compounds in biological fluids

Vaidyanath, N.; Birkhahn, R.; Border, J.R.; McMenamy, R.; Oswald, G.; Trietley, G.; Yuan, T.F.

Analytical Biochemistry 70(2): 479-488


ISSN/ISBN: 0003-2697
PMID: 1267142
DOI: 10.1016/0003-2697(76)90473-5
Accession: 000271963

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Centrifugal ultrafiltration was used to obtain plasma ultrafiltrates. Amino acids, including tryptophan, and NH3 were estimated automatically using 2 columns and split-stream methodology allowed estimation of radioactivities of amino acids, glucose and plasma water.