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Clinical studies on serum-cholinesterase in domestic animals. I. Measuring method of serum-cholinesterase activity in domestic animals


Clinical studies on serum-cholinesterase in domestic animals. I. Measuring method of serum-cholinesterase activity in domestic animals



Bulletin of the Faculty of Agriculture Kagoshima University (27): 115-121



In the DTNB (dithiobis nitrobenzoic acid) method, substrate sensitivity depended on the animal species - propionylthiocholine iodide (PTC) was most sensitive in horse, dog and cat serum, butyrylthiocholine iodide (BTC) and PTC were more reactive to monkey serum, and acetylthiocholine iodide (ATC) was most effective in pig and cattle serum. Optimum concentrations of substrate were 5 X 103 M/l in horse, monkey, dog and cat, 3 X 10-3 M/l in swine, and 1 X 10-3 in cattle.

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