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Comparative studies of two viroids: analysis of potato spindle tuber and citrus exocortis viroids by RNA fingerprinting and polyacrylamide-gel electrophoresis


Comparative studies of two viroids: analysis of potato spindle tuber and citrus exocortis viroids by RNA fingerprinting and polyacrylamide-gel electrophoresis



Virology 68(2): 309-316



ISSN/ISBN: 0042-6822

PMID: 1198921

DOI: 10.1016/0042-6822(75)90274-3

Highly purified isolates of PSTV from the tomato cv. Rutgers and CEV from Gynura aurantiaca were compared. Two dimensional fingerprinting analysis of PSTV RNA and CEV RNA labelled in vitro with 125I demonstrated that each of the RNA species has a complexity compatible with the size estimate of 250-350 nucleotides and that the RNA molecules do not have the same sequence. Polyacrylamide gel electrophoretic studies suggest, among other things, that there could be some sequence and/or structure heterogeneity within each of these viroid RNAs.

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Accession: 000320371

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