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Doubling chromosome numbers of wheat-rye amphiploids with colchicine, DMSO, and cold treatment

Doubling chromosome numbers of wheat-rye amphiploids with colchicine, DMSO, and cold treatment

Wheat Information Service (43): 10-12

Amphidiploid seedlings were obtained following culture of hybrid embryos derived from Triticum durum X rye and T. aestivum X rye, involving ten tetraploid and five hexaploid wheats and various diploid ryes. The main tiller of seedlings grown in pots, when 10 cm tall, was cut back to 2 cm from the crown and a vial containing 0.1% colchicine in an 2% aqueous solution of dimethyl sulphonate was inverted over the cut stem.

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Accession: 000342797

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