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Effect of varying combinations of time and pressure in acid hydrolysis of poultry excreta on the amino acid yield after separation by column chromatography



Effect of varying combinations of time and pressure in acid hydrolysis of poultry excreta on the amino acid yield after separation by column chromatography



Archiv fur Tierernahrung 25(3): 183-193



Excreta was hydrolysed with 6N HCl at 1, 2, 3, 4 or 5 kg/cm2 for 1, 2, 3, 4 or 5 h. Of 17 amino acids aspartic acid, valine, isoleucine, leucine and proline were stable and lysine, histidine, arginine, glutamic acid, glycine and alanine were semi-stable. The maximum release of amino acids occurred at 2 kg/cm2 for 5 h, 107 plus or minus 10% on average, compared with control hydrolysis at atmospheric pressure for 24 h; reducing the time to 4 h had a negligible effect.

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