Formation of sulfhydryl groups in the walls of Eimeria stiedai and E. tenella oocysts subjected to in vitro excystation

Jolley, W.R.; Burton, S.D.; Nyberg, P.A.; Jensen, J.B.

Journal of Parasitology 62(2): 199-202

1976


ISSN/ISBN: 0022-3395
PMID: 177746
DOI: 10.2307/3279265
Accession: 000383307

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Abstract
Eimeria stiedae or Eimeria tenella oocysts were incubated in aqueous cysteine (cysHCl) under carbon dioxide, aqueous cysHCl under air, water under CO2 or water under air, and analysed for sulphydryl (-SH) groups. The cysHCl-CO2 treatment produced more -SH groups than the other treatments and was effective in allowing activation of intact and sodium hypochlorite (NaOCl)-treated E. stiedae oocysts as well as NaOCl-treated E. tenella oocysts. The CO2-cysHCl complex may act directly on the oocyst wall, especially in the micropylar region, to unmask lipid-shielded disulphide bridges which are reduced to -SH groups. The reduction apparently disturbs the protein superstructure of the oocyst wall, promotes opening of the micropyle, and changes the impermeable state of the sporulated oocyst. [AS].