Formation of sulfhydryl groups in the walls of Eimeria stiedai and E. tenella oocysts subjected to in vitro excystation

Jolley, W.R.; Burton, S.D.; Nyberg, P.A.; Jensen, J.B.

Journal of Parasitology 62(2): 199-202


ISSN/ISBN: 0022-3395
PMID: 177746
DOI: 10.2307/3279265
Accession: 000383307

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Eimeria stiedae or Eimeria tenella oocysts were incubated in aqueous cysteine (cysHCl) under carbon dioxide, aqueous cysHCl under air, water under CO2 or water under air, and analysed for sulphydryl (-SH) groups. The cysHCl-CO2 treatment produced more -SH groups than the other treatments and was effective in allowing activation of intact and sodium hypochlorite (NaOCl)-treated E. stiedae oocysts as well as NaOCl-treated E. tenella oocysts. The CO2-cysHCl complex may act directly on the oocyst wall, especially in the micropylar region, to unmask lipid-shielded disulphide bridges which are reduced to -SH groups. The reduction apparently disturbs the protein superstructure of the oocyst wall, promotes opening of the micropyle, and changes the impermeable state of the sporulated oocyst. [AS].