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Haploid and diploid homozygotes of Nicotiana tabacum L., obtained by means of anther culture and tissue culture in vitro



Haploid and diploid homozygotes of Nicotiana tabacum L., obtained by means of anther culture and tissue culture in vitro



Hodowla Roslin, Aklimatyzacja i Nasiennictwo 20(5): 455-466



In anther culture of Hercegowina Pulawska 3800 (Hercegovina Pulawy 3800), 132 anthers gave rise to between one and 71 haploid plantlets each. These produced flowers with completely sterile pollen, were smaller than plants of the parental variety and in some cases differed also in morphology or colouring. Atempts to double the chromosome complement of haploid tissues cultured in vitro provided nine regenerated diploid plants (and one aneuploid) derived from the haploid I47 and four from II3.

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Accession: 000393161

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