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In vitro regeneration of shoot and root growth from meristematic tips of Pelargonium X hortorum Bailey


, : In vitro regeneration of shoot and root growth from meristematic tips of Pelargonium X hortorum Bailey. Acta Horticulturae (59): 131-141

A modification of the Murashige and Skoog tissue culture medium is described which enables the successful development of plantlets from P. meristem tips. In tests with the medium 32 P. hortorum cvs. were first exposed to thermotherapy (38 deg C for 4 weeks) and then cultured from meristem tips. After transplanting the cvs. were indexed on indicator plants for viruses reported to infect P.

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Related references

Horst R.K.; Smith S.H.; Horst H.T.; Oglevee W.A., 1976: In vitro regeneration of shoot and root growth from meristematic tips of pelargonium x hortorum. Acta Horticulturae (Wageningen) 59: 131-141

Witomska, M.; ukaszewska, A., 2004: Propagation of Pelargonium hortorum Bailey by axillary shoot formation in vitro. A method for the micropropagation of P. hortorum by axillary shoot formation in vitro was developed during 2001-02. In a first experiment, the effects of plant growth regulators (BA, benzyladenine at 0.2 mg/dm3 + NAA at 0.01 mg/dm3; and TDZ, thidi...

Hoelters, J.; Zimmer, K., 1990: Shoot regeneration from root tips of orchids in vitro ii. effect of shoot parts on root system and length and width of mormodes histrio roots on regeneration ability. Leaves inhibit formation of axillary shoots and shoot formation on roots of Mormodes histrio. Root borne shoots develop from protocorm like bodies [plb] only on isolated single roots or root systems without shoot. Formation of plb's depends o...

Heng, D.A.; Read, P.E., 1976: Influence of daminozide on the levels of root-promoting substances in Pelargonium hortorum Bailey. Fractionated ethanolic extracts from P. hortorum showed 1 major and 2 minor zones which promoted rooting of mung beans. The active fractions promoted rooting in the absence of IAA, in contrast to previous results. Levels of promoters fluctuated du...

Qureshi, J.A.; Saxena, P.K., 1992: Adventitious shoot induction and somatic embryogenesis with intact seedlings of several hybrid seed geranium (Pelargonium x hortorum Bailey) varieties. Intact seedlings of hybrid seed geranium (Pelargonium x hortorum Bailey) were tested for their ability to produce adventitious shoots and somatic embryos by direct culture of mature seeds on Murashige and Skoog (1962) medium (MS) supplemented with...

Qureshi, J.; Saxena, P., 1992: Adventitious shoot induction and somatic embryogenesis with intact seedlings of several hybrid seed geranium (Pelargonium X hortorum Bailey) varietes. Intact seedlings of hybrid seed geranium (Pelargonium X hortorum Bailey) were tested for their ability to produce adventitious shoots and somatic embryos by direct culture of mature seeds on Murashige and Skoog (1962) medium (MS) supplemented with...

Holters, J.; Zimmer, K., 1990: Shoot regeneration from root tips of orchids in vitro. II. Effect of shoot parts on the root system and of root length and width on regenerative ability of Mormodes histrio roots. Six-cm-long shoots with about 5 leaves and a root system were (1) defoliated, (2) decapitated, the leaves remaining, or (3) cut below the basal node, leaving only a root system with a small part of the shoot base, and cultured in vitro. In another...

Hoelters, J.; Zimmer, K., 1990: Shoot regeneration from root tips of orchids in vitro i. vegetative propagation of mormodes histrio in vivo and production of root tips. To conserve rare orchids, it is necessary to develop propagation methods. Using Mormodes histrio as a model plant it was tried to develop a method of in vitro propagation by root tips. At the first, starting with only two bulbous shoots of the pla...

Bigot, C., 1983: Vegetative propagation in vitro from seedlings of F1 hybrids of Pelargonium X hortorum Bailey. Acta Horticulturae: (131) 61-70

Anonymous, 2012: Ethylene and cytokinin interaction in the morphogenesis of Pelargonium × hortorum LH Bailey in vitro. <i>Pelargonium</i> <i> </i> <i>hortorum</i> Grand Prix which is susceptible to leaf yellowing and Bergpalais which is not susceptible to leaf yellowing were chosen for the experiments. Ethylene production and ac...