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Morphogenesis and plant regeneration from callus of immature embryos of sorghum


Morphogenesis and plant regeneration from callus of immature embryos of sorghum



Plant Science Letters 10(1): 67-74



DOI: 10.1016/0304-4211(77)90051-7

Callus with leafy shoots were obtained from immature embryos of sorghum (S. bicolor L. Moench) collected 12-18 days after pollination. The embryos were cultured on a defined agar medium containing Murashige-Skoog (MS) mineral salts supplemented with 5 .mu.M 2,4-D and 10-50 .mu.M zeatin. The callus was produced within 10 wk and further proliferated after subculture on the same medium. Small sections of callus with leafy shoots were transferred onto the same nutrient medium but containing 1-5 .mu.M IAA. Plantlets produced after 8-10 wk were transferred to pots and grown to maturity. Some plants were sterile; others produced fertile seeds. Meiotic metaphase examination of 25 panicles revealed chromosome number of 2n = 20 corresponding to normal plants with 10-10 anaphase distribution.

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Accession: 000434714

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