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On the determination of available lysine in casein and rapeseed protein concentrates using 2,4,6-trinitrobenzenesulphonic acid (TNBS) as a reagent for free epsilon amino group of lysine


On the determination of available lysine in casein and rapeseed protein concentrates using 2,4,6-trinitrobenzenesulphonic acid (TNBS) as a reagent for free epsilon amino group of lysine



Analytical Biochemistry 70(2): 434-439



ISSN/ISBN: 0003-2697

PMID: 1267137

DOI: 10.1016/0003-2697(76)90467-x

By establishing optimal conditions for the hydrolysis of TNP-labelled protein, a slight modification of the TNBS method of Kakade & Liener [Analytical Biochemistry (1969) 27, 273] for detn. of available lysine is proposed. The method involves the use of 30 mg samples and hydrolysis at 110 deg C for 90 min in sealed flasks. The changes substantially reduced the analytical variations, and provide a simple, rapid and more reproducible method. Results of 3 different methods for detn. of available lysine in casein and rapeseed protein concentrates are tabulated.

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Accession: 000448025

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