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Possibilities of improving detection of chrysanthemum stunt and obtaining viroid-free plants by meristem-tip culture

, : Possibilities of improving detection of chrysanthemum stunt and obtaining viroid-free plants by meristem-tip culture. Acta Horticulturae (59): 63-69

In studies on the improvement of chrysanthemum stunt viroid detection, special growing conditions combining high temps (25-28 deg C) and high light intensity (20 000 lux) allowed detection 20 days after inoculation. The results can be obtained with chrysanthemum Mistletoe or with Gynura aurantiaca. Two other cvs. (Bonnie Jean and Sunfire) are very susceptible to the disease and show symptoms in the glasshouse 15-20 days after inoculation.

Accession: 000463045

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Related references

Bachelier, J.; Monsion, M.D.nez, J., 1976: Possibilities of improving detection of chrysanthemum stunt and obtention of viroid free plants by meristem tip culture. Acta Horticulturae 59: 3-69

Horst, R.K.; Cohen, D., 1980: Amantadine supplemented tissue culture medium: a method for obtaining chrysanthemums free of chrysanthemum stunt viroid. Amantadine (50-100 mg/l) incorporated into basic Murashige and Skoog medium supplemented with 100 mg/l M-inositol, 0.4 mg/l thiamine, 30 g/l sucrose, 0.5 mg/l kinetin and 1.0 mg/l indolebutryic acid [IBA] was effective for obtaining chrysanthemum...

Wina Dian Savitri, Kyung I.Park…, 2013: Elimination of Chrysanthemum stunt viroid CSVd from meristem tip culture combined with prolonged cold treatment. Chrysanthemum production in Korea has recently been greatly affected by the spread of <em>Chrysanthemum stunt viroid</em> (CSVd) infection, necessitating the use of CSVd-free stocks to ensure successful chrysanthemum cultivation. We in...

Hosokawa, M.; Otake, A.; Ohishi, K.; Ueda, E.; Hayashi, T.; Yazawa, S., 2004: Elimination of chrysanthemum stunt viroid from an infected chrysanthemum cultivar by shoot regeneration from a leaf primordium-free shoot apical meristem dome attached to a root tip. In this research we eliminated chrysanthemum stunt viroid (CSVd) from a highly infected chrysanthemum cultivar using a newly established method. 'Piato' is one of the most difficult cultivars in which to obtain CSVd-free plants by conven...

Hotta, M.; Hasegawa, T.; Ohishi, K.; Kato, T.; Hosokawa, M., 2006: Effects of low temperature before or after acclimatization on growth and flowering of viroid-free chrysanthemum plants by leaf-primordia free shoot apical meristem culture and the variation test. On raising of viroid-free seedlings of chrysanthemum var. 'Jinba' and 'Iwanohakusen' by the new meristem culture method which leaf-primordia free Shoot apical is transplanted and cultured on in-vitro cabbage root tips, effects...

Arts, M.S.J.; Roenhorst, J.W.; Hooftman, R.; Zaayen, A. van, 1997: Routine detection of chrysanthemum stunt viroid in chrysanthemum plants by reverse transcription - polymerase chain reaction. The reverse transcription-polymerase chain reaction (RT-PCR) method was modified for the detection of chrysanthemum stunt viroid (CSVd) in chrysanthemum plants. This led to minor changes in the homogenization of leaf material, extraction method an...

Meldrais Y.A.; Line I.E.; Ivanova M.N.; Vinkele R.A.; Druka A.Ya; Gurinovich T.I., 1991: Chrysanthemum stunt viroid cdna cloning into plasmid puc19 and the cloned cdna for detection of chrysanthemum stunt viroid. 26 base long deoxyribonucleotide complementary to the lower part of the Central Conserved Region of chrysanthemum stund viroid (CSV) was used for synthesis of the first strand cDNA. The cDNA was cloned into plasmid vector pUC19 and the primary str...

Li, S.F.; Hataya, T.; Furuta, K.; Horita, H.; Sano, T.; Shikata, E., 1997: Occurrence of chrysanthemum stunt disease in Hokkaido and detection of chrysanthemum stunt viroid by electrophoresis and hybridization. In 1992, stunting and early flowering occurred in chrysanthemum cultivated in Naganuma, Yubarigun, Hokkaido, Japan. Chrysanthemum stunt viroid was detected from these plants by polyacrylamide gel electrophoresis (PAGE) and hybridization using a di...

Ghena N., 1970: Studying the possibilities of obtaining virus free strawberry plants by meristem culture. Proceedings of the International Horticultural Congress 1: 211

Nabeshima, T.; Doi, M.; Hosokawa, M., 2016: Agrobacterium-mediated inoculation of chrysanthemum (Chrysanthemum morifolium) plants with chrysanthemum stunt viroid. Agroinfiltration was tested as a method of inoculation of chrysanthemum plants with chrysanthemum stunt viroid (CSVd). Binary vectors harboring dimeric CSVd sequences in sense and antisense orientations were constructed, and Agrobacterium transfec...