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Prevention by polyvinylpyrrolidone of growth inhibition of Hamamelis shoot tips grown in vitro and of browning of the agar medium

, : Prevention by polyvinylpyrrolidone of growth inhibition of Hamamelis shoot tips grown in vitro and of browning of the agar medium. Acta Horticulturae (54): 101-104

Shoot tips of H. intermedia were cultured on a modified Murashige and Skoog's medium to which polyvinylpyrrolidone was added at 0.5, 1 or 2%. This compound prevents the polymerization of phenolic compounds. When it was present at 1% explant development was maximal and no browning occurred. The best results were obtained when shoot tips were taken in July (when the incidence of browning was low) rather than in May, June or August.

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