+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Prevention by polyvinylpyrrolidone of growth inhibition of Hamamelis shoot tips grown in vitro and of browning of the agar medium

Acta Horticulturae (54): 101-104

Prevention by polyvinylpyrrolidone of growth inhibition of Hamamelis shoot tips grown in vitro and of browning of the agar medium

Shoot tips of H. intermedia were cultured on a modified Murashige and Skoog's medium to which polyvinylpyrrolidone was added at 0.5, 1 or 2%. This compound prevents the polymerization of phenolic compounds. When it was present at 1% explant development was maximal and no browning occurred. The best results were obtained when shoot tips were taken in July (when the incidence of browning was low) rather than in May, June or August.

Accession: 000467431

Related references

Bader S.M.; Najim H.A.; Hummadi A.M., 1989: Effect of ph on the growth of shoot tips of date palm grown on murashige and skoog medium. Quarters of shoot tips of offshoots of zahdi cultivars were used as explants for this study. Five initial levels of pH values for Murashige and Skoog (MS) medium were examined. These values were 4.30, 5.00, 5.70, 6.40 and 7.10 (each level was refe...

Perez-Tornero, O.O.tin-Parraga, F.E.ea, J.B.rgos, L., 1999: Medium-term storage of apricot shoot tips in vitro by minimal growth method. Apricot (Prunus armeniaca L. cv. 'Helena') shoots grown on a proliferation medium containing 3% sucrose, 0.4 mg(.)L(-1) benzyladenine (BA), and 0.04 mg(.)L(-1) indolebutyric acid (BA) and solidified with 0.6% agar were stored at three di...

Niino T., 1990: Establishment of the cryopreservation procedure of mulberry shoot tips from in vitro grown shoot. The cryopreservation procedure of mulberry (Morus bombycis (cv. 'Kenmochi')) shoot tips excised from in vitro-grown shoot was developed. The appropriate procedure was shown as follows: The in vitro-grown shoots were hardened at 2.5-5.deg...

Chao-Hong Feng, Zhen-Hua Cui, Bai-Quan Li…, 2013: Duration of sucrose preculture is critical for shoot regrowth of in vitro-grown apple shoot-tips cryopreserved by encapsulation-dehydration. A simple and efficient cryopreservation protocol using encapsulation-dehydration was established for in vitro-grown shoot-tips of apple Gala (<i>Malus</i> <i>domestica</i> Borkh.). Shoot-tips, of 2.0 mm in length and with 5...

S.V.Kushnarenko, B.M.Reed, M.N.Normah, 2011: Cryopreservation of in vitro grown shoot tips. Shoot tip cryopreservation methods are well developed for many crops (Reed, 2008). These techniques especially work well for vegetatively propagated plants that cannot be stored as seeds (fruits, berries, and some vegetables). Techniques for stori...

Hrib J.; Chaturvedi H.C.; Dobry J., 1980: Development of shoot tips of sambucus sp grown in vitro. Shoot tips of Sambucus sp. were cultured on a modified Murashige and Skoog medium, with the addition of 0.1 mg l-1 benzylaminopurine. The in vitro grown shoots were transferred to a medium with macroelements of White and microelements of Heller. R...

Kuranuki, Y.; Sakai, A., 1994: Cryopreservation of in vitro-grown shoot tips of tea by vitrification. Cryobiology 31(6): 581

Hrib, J.C.aturvedi, H.; Dobry, J., 1981: Development of shoot tips of Sambucus sp. grown in vitro. Phytomorphology t (pub 1981) 30(2-3): 266-270

Phillips, D.J., 1964: Callus formation of carnation shoot tips grown in vitro. Growing pathogen-free plant material from shoot tips has become important in the control of carnation diseases. Abnormal and undesirable callus formation on shoot tips in vitro has limited success in producing mature plants. Shoots tips were excis...

Niino, T.; Sakai, A.; Enomoto, S.; Magosi, J.; Kato, S., 1992: Cryopreservation of in vitro-grown shoot tips of mulberry by vitrification. In vitro-grown shoot tips of mulberry (Morus bombycis Koidz. cv. Kenmochi) successfully cryopreserved by vitrification. Three-week-old in vitro mulberry plantlets were cold-hardened at 5 degree C for 3 weeks. Excised shoot tips from hardened plant...