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Purification of carnation etched ring virus and comparative properties of CERV and cauliflower mosaic virus nucleic acids


Acta Horticulturae (59): 49-59
Purification of carnation etched ring virus and comparative properties of CERV and cauliflower mosaic virus nucleic acids
CERV was partially purified from mechanically inoculated Saponaria vaccaria Pink Beauty leaves by butanol clarification, alone or with urea and triton X-100. Infectivity was associated with a major UV absorbing band c. 12-17 mm below the meniscus and in several fractions adjacent to the band in a 10-40% sucrose density gradient. CERV nucleic acid (NA) consists predominantly of linear molecules 0.12 - >35 mu m long, mostly <1 mu m.


Accession: 000474167



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