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Purification of carnation etched ring virus and comparative properties of CERV and cauliflower mosaic virus nucleic acids


, : Purification of carnation etched ring virus and comparative properties of CERV and cauliflower mosaic virus nucleic acids. Acta Horticulturae (59): 49-59

CERV was partially purified from mechanically inoculated Saponaria vaccaria Pink Beauty leaves by butanol clarification, alone or with urea and triton X-100. Infectivity was associated with a major UV absorbing band c. 12-17 mm below the meniscus and in several fractions adjacent to the band in a 10-40% sucrose density gradient. CERV nucleic acid (NA) consists predominantly of linear molecules 0.12 - >35 mu m long, mostly <1 mu m.

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