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Purification of carnation etched ring virus and comparative properties of CERV and cauliflower mosaic virus nucleic acids


, : Purification of carnation etched ring virus and comparative properties of CERV and cauliflower mosaic virus nucleic acids. Acta Horticulturae (59): 49-59

CERV was partially purified from mechanically inoculated Saponaria vaccaria Pink Beauty leaves by butanol clarification, alone or with urea and triton X-100. Infectivity was associated with a major UV absorbing band c. 12-17 mm below the meniscus and in several fractions adjacent to the band in a 10-40% sucrose density gradient. CERV nucleic acid (NA) consists predominantly of linear molecules 0.12 - >35 mu m long, mostly <1 mu m.


Accession: 000474167

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Related references

Hull, R.; Sadler, J.; Longstaff, M., 1986: The sequence of carnation etched ring virus DNA: comparison with cauliflower mosaic virus and retroviruses. Carnation etched ring virus (CERV) DNA comprises 7932 bp. CERV primer binding sites and overall genome organization are similar to those of the related cauliflower mosaic virus (CaMV). The six open reading frames of CERV showed amino acid homology...

Lawson, R.H.; Civerolo, E.L., 1978: Carnation etched ring virus purification stability of inclusions and properties of the nucleic acid. Carnation etched ring virus (CERV) was partially purified from mechanically inoculated fresh leaf tissue of Saponaria vaccaria 'Pink Beauty.' The yield of CERV from crude sap leaf extracts or from partially purified and concentrated CERV...

Abdulmagid, A.M.; Robb, S.M., 1987: The effect of carnation etched ring virus (CERV) on growth and flower yield of Doris variety of pinks (Dianthus sp.). Plants of this Dianthus cultivar freed from virus infection by meristem tip culture gained substantially in vigour, number of lateral shoots, total number of flowers, flower quality, fresh wt and other desirable characters.

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B.R.K.luev; A.V.K.yazev; A.V.C.emeris, 2008: Activity of promoters of carnation etched ring virus and dahlia mosaic virus in tobacco protoplasts and transgenic plants. Russian Journal of Plant Physiology 55(5): 687-693

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