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Purification of mouse pepsinogens by pepstatin-affinity chromatography

Purification of mouse pepsinogens by pepstatin-affinity chromatography

FEBS Letters 86(1): 33-36

ISSN/ISBN: 0014-5793

PMID: 620824

DOI: 10.1016/0014-5793(78)80092-1

2 forms of pepsinogen (PgI and PgII) from the gastric mucosa of mice were purified to homogeneity using pepstatin/Sepharose 4B affinity chromatography, gel filtration and Sephadex G-100 and DEAE/Sepharose CL6B column chromatography. After activation at pH 2.2, PgI and PgII showed milk-clotting activity at pH 5.3. The PgI showed this activity in 60 s at 37 deg C and PgII in 67 s. There was no activity without activation.

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Accession: 000474189

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