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Regeneration of plants from anther cultures of sweet potato (Ipomoea batatas Poir.)



Regeneration of plants from anther cultures of sweet potato (Ipomoea batatas Poir.)



Zeitschrift fur Pflanzenphysiologie 88(4): 349-352



Anthers of I. batatas Poir. (= Batatas edulis Choisy) excised at various stages of pollen development and cultured on Murashige and Skoog's medium (MS) supplemented with 1 or more of the growth regulators, Ad [adenine], Kn [kinetin] Ze [zeatin], IAA, NAA [naphthalene acetic acid] and 2,4-D, failed to produce androgenic haploids, while those cultured on MS + Ad (10, 20 ppm) + 2,4-D (1 ppm) produced a profuse callus from anther wall as well as the connective tissue. The callus of such a dual-tissue origin retained its diploidy through all the passages. Upon transfer to MS the callus gave rise to diploid plantlets.

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Accession: 000479102

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DOI: 10.1016/S0044-328X(78)80137-8


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