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Serum protein fractions from children of differing nutritional status analysed by polyacrylamide gel electrophoresis and electroimmunoassay

Serum protein fractions from children of differing nutritional status analysed by polyacrylamide gel electrophoresis and electroimmunoassay

British Journal of Nutrition 35(2): 211-222

The nutritional status of children showing no clinical signs of malnutrition, from the University School of Khon Kaen, Khon Kaen province, N-E Thailand, and from 2 villages nearby, was tested. The children were grouped according to their body-weight expressed as a percentage of expected weight-for-height (Harvard standards (Stuart and Stevenson, 1959), as given by Jelliffe (1966)). The differing prealbumin concentrations indicated that nutritional status differed between the groups. The urinary urea:creatinine ratio was significantly lower in the village children compared with the children from Khon Kaen, indicative of the higher dietary protein intake of the latter. .alpha.1-Acid glycoprotein and the 1st post-albumin peak (obtained by polyacrylamide gel electrophoresis of serum and containing mainly Gc-globulin, .alpha.1-antichymotrypsin and .alpha.1-B-glycoprotein) were found to be significantly higher in the village children compared with children from Khon Kaen. The 3 main proteins of the 1st post-albumin peak from polyacrylamide gel electrophoresis of serum were tested separately using the electroimmunoassay method. There was no significant difference in Gc-globulin between the children from Khon Kaen and the village children. The concentration of .alpha.1-B-glycoprotein from those Khon Kaen children whose bodyweight was more than 95% expected weight-for-height was significantly lower compared with that of village children. .alpha.1-Antichymotrypsin concentration was significantly higher in serum from Khon Kaen children than in serum from village children.

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Accession: 000496120

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PMID: 56197

DOI: 10.1079/bjn19760025

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