Studies on Pneumocystis carinii and Pneumocystis carinii pneumonia. I. Evaluation of several kinds of staining method in the identification of P. carinii

Ogino, K.; Yoshida, Y.; Takeuchi, S.; Ikai, T.; Yamada, M.

Japanese Journal of Parasitology 26(3): 116-125


Accession: 000509417

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A combination of intracystic body staining and cyst wall staining is required to confirm the diagnosis of Pneumocystis carinii pneumonia in lung smears from rats and humans. Giemsa and Toluidine blue-O are recommended for these 2 staining purposes, respectively. A combination of HE stain and Toluidine blue-O stain is recommended to show the host reaction and the parasite cyst wall, respectively, in lung sections.