Synergistic action of red and blue light and action spectra for malate formation in guard cells of Vicia faba L

Ogawa, T.; Ishikawa, H.; Shimada, K.; Shibata, K.

Planta 142(1): 61-65


ISSN/ISBN: 0032-0935
PMID: 24407999
DOI: 10.2307/23373493
Accession: 000520562

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Malate formation in guard cells o- V. faba leaves is enhanced by light. The action spectrum for this effect was determined for epidermal strips of V. faba, and 2 different spectra were obtained under different light conditions and with and without background irradiation with high-irradiance red light (> 600 nm, 3.0 mW cm-2) superimposed on monochromatic light of other wavelengths. The spectrum obtained at quantum fluxes of 1.7-2.2 nE cm-2 .cntdot. s-1 of monochromatic light without background red light showed a sharp peak at 433 nm with a shoulder around 475 nm and a lower peak at 670-680 nm; the spectrum obtained at much lower quantum fluxes of 0.05-0.07 nE cm-2 .cntdot. s-1 of monochromatic light with red-light background had 2 peaks of comparable heights at 380 and 460 nm. The formation of malate with 430 nm blue light was saturated at a quantum flux of 3 nE cm-2 .cntdot. s-1 without the background red light but at a much lower quantum flux of 0.2 nE cm-2 .cntdot. s-1 with the background red light. At this low intensity, blue light was practically ineffective without background red light. A synergistic action of red light presumably absorbed by the chlorophylls, and blue light absorbed by a yellow pigment is thus demonstrated by these experiments. The action maxima at 380 and 460 nm for the blue-light effect in the presence of background red light agree with the absorption maxima of flavins.