The demonstration in vitro of the mitogenic effects of trypanosomal antigen on the spleen cells of normal athymic and cyclophosphamide-treated mice
Clinical and Experimental Immunology 23(2): 314-317
ISSN/ISBN: 0009-9104 Accession: 000529828
When the spleen cells of normal NIH mice were cultured with pokeweed mitogen (PWM), staphylococcal filtrate (SF) or an antigen (TAg) derived from Trypanosoma brucei TREU 226, and tritiated thymidine (dr) incorporation was used as a measure of mitogenic activity, the TAg (at a level of 25 mu g/ml of spleen cell suspensions containing 2 to 3 X 106 cells/ml) was found to be a better mitogen than SF. PWM, however, was more effective than either of the two.