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Use of a Coulter Counter to detect discrete changes in cell numbers and volume during growth of Escherichia coli

Use of a Coulter Counter to detect discrete changes in cell numbers and volume during growth of Escherichia coli

Journal of Applied Bacteriology 39(2): 157-165

E. coli could be detected at a concentration of 105 cells/ml. Growth curves of the organism grown in filtered tryptone soya broth were made by taking viable and Coulter counts. Both curves were similar in shape and varied little during 10 h growth. Cell volume and volume distribution were seen to vary considerably, however, volume reaching a stable minimum value of 0.61 mu m3 after 8 h. The Coulter Counter was considered to be potentially useful for detecting significant bacteriuria.

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Accession: 000568227

Download citation: RISBibTeXText

PMID: 1104554

DOI: 10.1111/j.1365-2672.1975.tb00557.x

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