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Use of spin labels and electron spin resonance spectroscopy to characterize membranes of bovine sperm: effect of butylated hydroxytoluene and cold shock



Use of spin labels and electron spin resonance spectroscopy to characterize membranes of bovine sperm: effect of butylated hydroxytoluene and cold shock



Biology of Reproduction 14(4): 381-397



For spermatozoa suspended in aqueous medium, the cold shock associated with rapid cooling from room temp. to 0 deg C increased membrane permeability. This membrane damage was readily detected using spin labels, but was not detected using thin-section electron microscopy. This change in the membrane was prevented by the addition of butylated hydroxytoluene (BHT). BHT also provided partial protection against further damage caused by freezing spermatozoa on solid CO2.

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Accession: 000569354

Download citation: RISBibTeXText

PMID: 179626

DOI: 10.1095/biolreprod14.4.381


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