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A new technique for observing mitotic chromosomes, beginning with protoplasts


, : A new technique for observing mitotic chromosomes, beginning with protoplasts. Acta Horticulturae: 57-60

Mitotic protoplasts were subjected to hypotonic shock, then spread on a slide.


Accession: 000585073

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Related references

Malmberg R.L.; Griesbach R.J., 1980: The isolation of mitotic and meiotic chromosomes from plant protoplasts. A method was developed for the isolation of whole chromosomes from plant protoplasts of both mitotic and meiotic cells. Mitotic chromosomes were isolated from protoplasts taken from synchronized liquid suspension cultures of both Nicotiana tabacum...

Fredga, Karl, 1964: A simple technique for demonstration of the chromosomes and mitotic stages in a mammal Chromosomes from cornea. The technique depends on the high mitotic activity in the corneal epithelium. After killing the animal, the eyes are dissected out and placed into 0.7% Na-citrate solution of 37[degree]C. After 30 min hypotonic treatment at 37[degree]C, the eyes a...

Stushnoff, C.; Feliciano, A.J., 1968: A simple technique for observing mitotic division of the generative nucleus in pollen tubes of Vaccinium spp. The technique involves distributing pollen evenly on the surface of a medium and after sealing, culturing at 20 degrees C. Hourly samples are stained with 1 g. crystal violet refluxed in 100 ml. 45% acetic acid for three hours and observed under t...

Ghazizadeh, M.; Sasaki, Y.; Oguro, T.; Sato, S.; Egawa, S.; Inoue, K.; Adachi, A.; Shimizu, H.; Kawanami, O., 2008: A novel technique for observing the internal ultrastructure of human chromosomes with known karyotype. Observation of the internal ultrastructure of human chromosomes by transmission electron microscopy (TEM) has frequently been attempted in spite of the difficulties in detaching metaphase chromosome,spreads from the glass slide for further proces...

Tilquin, J.; Dujardin, M.L.uant, B., 1979: Use of Giemsa C-banding technique for observing chromosomes of Cichorium intybus L. Annales de l'amelioration des plantes9(4): 419-425

Tilquin J P.; Dujardin M.; Louant B P., 1979: Use of giemsa c banding technique for observing chromosomes of cichorium intybus. The Giemsa staining technique was used to produce heterochromatic C-bands in the somatic metaphase chromosomes of C. intybus L. Centromeric, telomeric and intercalary bands are obtained in the 9 pairs and the homologous chromosomes are identified...

Mouras, A.; Salesses, G.; Lutz, A., 1978: Protoplasts use in cytology improvement of recent method in order to identify nicotiana and prunus mitotic chromosomes. Plant protoplasts treated in the same way as lymphocytes in animal cytogenetics pointed out a chromosome counting technique in tobacco [N. tabacum] tissue cultures. The analysis of the factors important to develop this method (pretreatment, osmoti...

Van Buijtenen, J.P., 1959: A technique for fixing and staining mitotic chromosomes in aspen. A routine aceto-carmine method suitable for obtaining approximate chromosome counts has been successfully used in distinguishing triploid from diploid aspen.

Hübner, H.; Kononowicz, A.K., 1977: Autoradiographic studies on differentiation of chromatin in interphase nuclei and mitotic chromosomes. II. Incorporation of 3-h-thymidine and binding of 3h-actinomycin D by selected segments of human mitotic chromosomes as a function of their differentiated ability to bind acridine fluorochrome. Acta Medica Polona 18(3): 251-260

Mogford, D.J.; Vosa, C.G., 1980: A fluorescence technique for the detection of knobs in the mitotic chromosomes of maize. This is an expanded account of information noticed elsewhere.