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Conversion of high molecular weight CRF (corticotropin-releasing factor) or PRF (prolactin-releasing factor) into lower molecular weight forms by boiling at low pH

Conversion of high molecular weight CRF (corticotropin-releasing factor) or PRF (prolactin-releasing factor) into lower molecular weight forms by boiling at low pH

Biochemical and Biophysical Research Communications 85(4): 1291-1298

ISSN/ISBN: 0006-291X

PMID: 33676

DOI: 10.1016/0006-291x(78)91143-9

Extracts (0.1 N HCl) of bovine hypophysial stalk had 2 CRF peaks, one (CRF-A) in the void vol. with Sephadex G-100 chromatography and the other more retarded (CRF-B). The PRF activity of the same extracts eluted in 2 peaks from G-100, one in the void vol. (PRF-A) and the other (PRF-B) between A and B CRF peaks. On rechromatography, isolated CRF-A and PRF-A remained in the void vol. However, heating to 100 deg C at pH 1-2 for 15 min converted CRF-A to CRF-B and PRF-A to PRF-C, which eluted after PRF-B on G-100. It was concluded that CRF or PRF can be converted from high to low mol. wt. forms with full retention of biological activity.

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Accession: 000623143

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