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Detection by immunofluorescence of transmissible gastroenteritis (TGE) viral antigen in pigs, using cat anti feline infectious peritonitis (FIP) virus conjugate

Detection by immunofluorescence of transmissible gastroenteritis (TGE) viral antigen in pigs, using cat anti feline infectious peritonitis (FIP) virus conjugate

Zentralblatt fur Veterinarmedizin 25B(9): 775-778

Sequential sections through intestinal specimens from 218 pigs, from 120 TGE suspect farms, were examined; anti-FIP and anti-TGE virus conjugates were used in parallel. Sixty-one of the specimens examined contained TGE viral antigen as demonstrated by the anti-TGE virus conjugate. The same sections also showed fluorescence with the anti-FIP virus conjugate. Preparations negative in the homologous reaction were also negative in the heterologous test. Intensity and localization of the fluorescence with both conjugates in subsequent sections of the same preparations were comparable. The anti-FIP virus conjugate working dilution (1:20) was four times higher than the anti-TGE virus conjugate dilution. The results confirm the antigenic relationship between TGE and FIP viruses. Absence of fluorescence in FIP virus-positive spleen sections using an anti-TGE virus conjugate confirms the evidence for the one-way antigenic relationship reported in the literature.

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Accession: 000628582

Download citation: RISBibTeXText

PMID: 369240

DOI: 10.1111/j.1439-0450.1978.tb01072.x

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