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Determination of ethanol content in culture media of lactic streptococci



Determination of ethanol content in culture media of lactic streptococci



Trudy, Vsesoyuznyi Nauchno issledovatel' skii Institut Maslodel' noi i Syrodel' noi Promyshlennosti Nauchno proizvodstvennogo Ob"edineniya ' Uglich' (24): 37-39, 106



25 ml culture medium are made slightly alkaline with 1 N NaOH and reduced by distillation to 5 ml, the distillate being collected. 10 ml 0.005% K2Cr2O7 solution in 28% HNO3 are added to 1 ml distillate; the reaction mixture is held for 2 h at 50 deg C, the intensity of the colour is measured at 440 nm, and quantity of ethanol is read from a standard empirically established curve. The method is suitable for detection of ethanol levels of 10-100 mg/100 ml.

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Accession: 000629058

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