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Experiments on the production of virus free raspberry propagation material by meristem culture

, : Experiments on the production of virus free raspberry propagation material by meristem culture. Acta Horticulturae (95): 77-78

Shoot tips of stratified and forced raspberry roots were disinfected in chloramine, and meristem tips with 1-2 leaf primordia cut out and placed in an agar gel medium containing macroelements (Morel), microelements (Heller), several B vitamins (1 p.p.m.), myoinositol (100 mg/l), glucose (2-3%) and indolyl butyric acid (0.1 p.p.m.). Rooting was induced using the same medium with a lowered auxin conc. Rooted plantlets could be dissected for further propagation or transplanted into soil.

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Related references

Sobczykiewicz, D., 1983: Meristem culture for the production of virus-free raspberry plants. An improved technique for proliferation and rooting of raspberry plants obtained from meristems is described. About 80% of the plants showed no visible symptoms of virus infection.

Putz, C., 1971: Production of virus-free Raspberry plants (var. Bois Blanc) by meristem culture. The best method was the culture of dissected apices (0.2-0.6 mm) from shoots infected with raspberry mosaic virus on a Murashige and Skoog (MS) medium+ 1 ppm gibberellin, followed 4 weeks later by transfer to a MS + 1 ppm kinetin medium.

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