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Experiments on the production of virus free raspberry propagation material by meristem culture

Acta Horticulturae (95): 77-78

Experiments on the production of virus free raspberry propagation material by meristem culture

Shoot tips of stratified and forced raspberry roots were disinfected in chloramine, and meristem tips with 1-2 leaf primordia cut out and placed in an agar gel medium containing macroelements (Morel), microelements (Heller), several B vitamins (1 p.p.m.), myoinositol (100 mg/l), glucose (2-3%) and indolyl butyric acid (0.1 p.p.m.). Rooting was induced using the same medium with a lowered auxin conc. Rooted plantlets could be dissected for further propagation or transplanted into soil.

Accession: 000657357

Related references

Sobczykiewicz, D., 1983: Meristem culture for the production of virus-free raspberry plants. An improved technique for proliferation and rooting of raspberry plants obtained from meristems is described. About 80% of the plants showed no visible symptoms of virus infection.

Putz, C., 1971: Production of virus-free Raspberry plants (var. Bois Blanc) by meristem culture. The best method was the culture of dissected apices (0.2-0.6 mm) from shoots infected with raspberry mosaic virus on a Murashige and Skoog (MS) medium+ 1 ppm gibberellin, followed 4 weeks later by transfer to a MS + 1 ppm kinetin medium.

Vertesy, J., 1976: Production of virus free material of some strawberry varieties by tip meristem culture

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Houten, J.G.; Quak, F.; Meer, F.A.V.n Der, 1968: Heat treatment and meristem culture for the production of virus-free plant material. To obtain virus-free material from plant species or cultivars which are vegetatively propagated and totally infected with virus, 3 methods were developed. Heat treatment (with hot water or hot air) resulting in an inactivation or an inhibition of...

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Mikhovska, B.; Petkov, T.; Dragoiski, K.; Stoevska, T., 1995: Obtaining and propagation of virus-free planting material of raspberry. Since 1991 at the Institute of Upland Stock Breeding and Agriculture, trials have been conducted for obtaining, maintaining and propagating virus-free raspberry planting material. By means of phytosanitary selection, typical plants of 4 Bulgarian...

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