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Functional characteristics of intact chloroplasts isolated from mesophyll protoplasts and bundle sheath cells of maize



Functional characteristics of intact chloroplasts isolated from mesophyll protoplasts and bundle sheath cells of maize



Planta 141(3): 239-244



A procedure was developed to isolate maize mesophyll and bundle sheath chloroplasts of a high degree of intactness and low cross-contamination. Light-induced 14CO2 fixation of isolated chloroplasts was similar to that of protoplasts and cells in that it was low and stimulated by the addition of exogenous substrates. O2 evolution was absent in both bundle sheath chloroplasts and cells.

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Accession: 000664867

Download citation: RISBibTeXText

PMID: 24414867

DOI: 10.1007/bf00388338


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