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Haemolymph ecdysone concentrations in Hyalophora cecropia pupae, dauer pupae and adults

Haemolymph ecdysone concentrations in Hyalophora cecropia pupae, dauer pupae and adults

Journal of Insect Physiology 252: 143-145

Hemolymph ecdysone concentrations were determined by radioimmunoassay in diapausing pupae, pharate adults, adults and chilled dauer pupae. The concentration in diapausing pupae after 6 mo. chilling (5.35 pg/.mu.l) increased dramatically after 3 days at 27.degree. C (> 200 pg/.mu.l) and then decreased to low levels in adult females (1.63 pg/.mu.l). In adult males ecdysone was undetectable in all except 1 animal. Dauer pupae showed a decrease from 6.1 to 1.7 pg/.mu.l 1 day after being transferred from 6 to 27.degree. C. Over 3 days the value increased to 3.19 pg/.mu.l and remained constant for > 1 yr. Diapausing pupae with and without brain neurosecretory cells maintain a low concentration of ecdysone in the hemolymph.

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Accession: 000670974

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DOI: 10.1016/0022-1910(79)90092-1

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