Section 1
Chapter 673

High performance liquid chromatographic determination of vitamin D in fortified dried milks

Furuta, K.; Adachi, A.; Kobayashi, T.

Vitamins 52(9/10): 409-415


Accession: 000672956

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A routine HPLC method is described. Fat is extracted and alkali-saponified, non-saponifiable matter is isolated, purified with digitonin-Celite and Florex-XXS column chromatography then by TLC of the benzene Florex fraction, and then vitamin D is determined by HPLC on a Zorbax SIL column. Samples from dried milks commercially fortified with vitamin D were co-chromatographed with authentic vitamin D2; a single peak resulted, confirming that vitamin D2 is used for commercial fortification.

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