Immunological investigations of house-dust and house-dust mites. II. Localization of the antigen in the body of the house-dust mite Dermatophagoides pteronyssinus by means of the indirect immuno-fluorescence method

Mumcuoglu, Y.; Rufli, T.

Recent Advances in Acarology Volume II: 205-210


Accession: 000676830

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Laboratory tests were carried out in Switzerland to investigate the antigenic components of Dermatophagoides pteronyssinus (Trt.), using indirect immunofluorescence. Rabbits were sensitised against mite extracts in order to obtain hyperimmune sera, and mites were embedded in methacrylate and cut into sections 3 mu m thick to be used as the antigenic substrate. It was demonstrated that the antigen is partly localised in the cuticle but mainly in the intestinal tract of the mite, the food being transformed in the intestinal tract and excreted as a strong antigen. Sections of mites incubated with the sera from rabbits sensitised against the culture medium (dander-yeast mixture) showed a weak fluorescence in the intestinal region.