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Immunological investigations of house-dust and house-dust mites. II. Localization of the antigen in the body of the house-dust mite Dermatophagoides pteronyssinus by means of the indirect immuno-fluorescence method



Immunological investigations of house-dust and house-dust mites. II. Localization of the antigen in the body of the house-dust mite Dermatophagoides pteronyssinus by means of the indirect immuno-fluorescence method



Recent Advances in Acarology Volume II: 205-210



Laboratory tests were carried out in Switzerland to investigate the antigenic components of Dermatophagoides pteronyssinus (Trt.), using indirect immunofluorescence. Rabbits were sensitised against mite extracts in order to obtain hyperimmune sera, and mites were embedded in methacrylate and cut into sections 3 mu m thick to be used as the antigenic substrate.

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