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Inactivated-concentrated virus antigen for indirect complement fixation test of foot-and-mouth disease



Inactivated-concentrated virus antigen for indirect complement fixation test of foot-and-mouth disease



National Institute of Animal Health Quarterly 18(3-4): 128-134



To the culture fluids of BHK-21 cells infected with each of types O, A, and Asia 1 of FMD virus was added acetylethyleneimine to 0.05% (v/v). The mixtures were incubated at 37 deg C for 24 hours. Polyethylene glycol 6000 was then added to 10% (w/v), and the mixtures concentrated to one-tenth of the initial volume. The resulting inactivated-concentrated virus antigens showed a CF titre ranging from 12 to 24. The recovery rate of CF activity was 40-60%.

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Accession: 000678584

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PMID: 216926


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