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Plasmodium gallinaceum: transmission-blocking immunity in chickens. II. The effect of antigamete antibodies in vitro and in vivo and their elaboration during infection

Plasmodium gallinaceum: transmission-blocking immunity in chickens. II. The effect of antigamete antibodies in vitro and in vivo and their elaboration during infection

Experimental Parasitology 47(2): 194-208

Heat-inactivated serum from chickens with transmission-blocking immunity to Plasmodium gallinaceum prevented the in vitro development of ookinetes from gametocytes of P. gallinaceum only when present during the period between the initiation of gametogenesis and the release of the microgametes. When added after this time immune serum failed to suppress ookinete development. Immune serum did not prevent the formation of gametes from gametocytes. These results are interpreted to indicate that immune serum contains factors which prevent fertilization of the malarial gametes but which do not affect the development of the zygote once fertilization has taken place. Two distinct reactions of malarial gametes with serum from chickens with transmission-suppressing immunity are described-the gamete-agglutination (AG) reaction and the microgamete surface-fixation (SF) reaction. Both reactions were associated with the immunoglobulin fraction of immune serum. The presence of SF antibodies during a blood infection correlated closely with effective transmission-blocking immunity in vivo; AG antibodies, on the other hand, were present in various circumstances in the absence of transmission-blocking immunity. AG and SF antibodies occurred not only in birds immunized with P. gallinaceum-gamete preparations but also during or following infections in unimmunized birds; SF antibodies appeared only following the peak of asexual infection in unimmunized birds and were of low titre. In immunized birds blood infections following live challenge invariably boosted low levels of SF antibodies. [AS].

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Accession: 000719787

Download citation: RISBibTeXText

PMID: 556175

DOI: 10.1016/0014-4894(79)90073-0

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