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31P NMR studies of metabolism in Acanthamoeba castellanii: polyphosphate release from encysted cells



31P NMR studies of metabolism in Acanthamoeba castellanii: polyphosphate release from encysted cells



Biochemical and Biophysical Research Communications 953: 1211-1217



Encysting cells of Acanthamoeba castellanii were shown, by 31P NMR studies, to release polyphosphate into the medium. Mature cysts and vegetative cells contained low levels of polyphosphatase. Young cysts (7 days) showed detectable levels of nucleotide diphosphates and triphosphates similar to those observed in vegetative cells. Mature cysts (90 days) showed only excreted polyphosphate as well as a component which had a chemical shift of a phosphodiester. The inorganic phosphate peak in the cyst showed that the cyst milieu is liquid-like and that the intracellular environment maintains a pH between 6.0 and 7.5 in the presence of extracellular values from 4.0 to 9.0. [AS].

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Accession: 000803697

Download citation: RISBibTeXText

PMID: 7417309

DOI: 10.1016/0006-291x(80)91602-2


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