+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

31P NMR studies of metabolism in Acanthamoeba castellanii: polyphosphate release from encysted cells

31P NMR studies of metabolism in Acanthamoeba castellanii: polyphosphate release from encysted cells

Biochemical and Biophysical Research Communications 953: 1211-1217

Encysting cells of Acanthamoeba castellanii were shown, by 31P NMR studies, to release polyphosphate into the medium. Mature cysts and vegetative cells contained low levels of polyphosphatase. Young cysts (7 days) showed detectable levels of nucleotide diphosphates and triphosphates similar to those observed in vegetative cells. Mature cysts (90 days) showed only excreted polyphosphate as well as a component which had a chemical shift of a phosphodiester. The inorganic phosphate peak in the cyst showed that the cyst milieu is liquid-like and that the intracellular environment maintains a pH between 6.0 and 7.5 in the presence of extracellular values from 4.0 to 9.0. [AS].

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 000803697

Download citation: RISBibTeXText

PMID: 7417309

DOI: 10.1016/0006-291x(80)91602-2

Related references

Phosphorus 31 nmr studies of metabolism in acanthamoeba castellanii poly phosphate release from encysted cells. Biochemical & Biophysical Research Communications 95(3): 1211-1217, 1980

31PNMR studies of vegetative and encysted cells of Acanthamoeba castellanii Observation of phosphonic acids in live cells. European Journal of Biochemistry 1112: 369-375, 1980

Phosphorus 31 nmr studies of vegetative and encysted cells of acanthamoeba castellanii observation of phosphonic acids in live cells. European Journal of Biochemistry 111(2): 369-376, 1980

Magnetic resonance studies of structural and metabolic properties of acanthamoeba castellanii in vegetative and encysted states. Federation Proceedings 39(6): ABSTRACT 2679, 1980

Electron microscopic study of species of the acanthamoeba castellanii group acanthamoeba castellanii acanthamoeba rhysodes acanthamoeba polyphaga. Rivista di Parassitologia 39(2-3): 49-62, 1978

Observation by 13C NMR of metabolites in differentiating amoeba: trehalose storage in encysted Acanthamoeba castellanii. FEBS Letters 118(2): 185-190, 1980

Acanthamoeba castellanii trophozoites use ADP-release as strategic arm to kill target cells. Journal of Eukaryotic Microbiology 48(1): 24A, January-February, 2001

Carbohydrate metabolism in Acanthamoeba castellanii. I. The activity of key enzymes and (14C)-glucose metabolism. Canadian Journal of Microbiology 19(9): 1131-1136, 1973

Oxidative metabolism associated with phagocytosis in Acanthamoeba castellanii. Journal of Protozoology 32(2): 330-333, 1985

Mannose induces the release of cytopathic factors from Acanthamoeba castellanii. Infection and Immunity 66(1): 5, 1998

Polyamine metabolism and its relationship to multiplication and differentiation of Acanthamoeba castellanii. Dissertation Abstracts International B Sciences and Engineering, 4710: 4057, 1987

Induction of differentiation in acanthamoeba castellanii by inhibitors of polyamine metabolism. Journal of Cell Biology 99(4 Part 2): 242A, 1984

Inositol lipid metabolism and phagocytosis in the protozoan acanthamoeba castellanii. Journal of Cellular Biochemistry Suppl. (11 Part B): 162, 1987

Enzymes of phospholipid metabolism in the plasma membrane of Acanthamoeba castellanii. Journal of Lipid Research 16(1): 54-60, 1975

Studio microelettronico di Acanthamoeba castellanii-group spp.: A. castellanii, A. rhysodes, A. poliphaga. Rivista di Parassitologia, 392-3: 49-62, 1978