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An evaluation of mineral oil seal for preservation of basidiomycetes cultures

An evaluation of mineral oil seal for preservation of basidiomycetes cultures

Weishengwu Xuebao 21(1): 45-52

The viabilities of 161 strains belonging to 63 spp. of 44 genera of Basidiomycetes preserved in mineral oil for 5-8 yr were examined. The results of the viability tests of these cultures are as follows: of the 87 strains from 43 spp., 83 strains (94.4%) are viable for 8 yr; of the 124 strains from 52 spp., 124 strains (100%) for 7 yr; of the 142 strains from 57 spp., 139 strains (97.9%) for 6 yr; of the 161 strains from 63 spp., 155 strains (95.8%) for 5 yr. Among these cultures only Ganoderma japonicum (8 years) and some Agaricus pratensis strains (6 and/or 5 yr) lost their viability. When 27 strains from 16 spp., preserved in mineral oil for 5-8 yr, were checked for the ability to form fruit bodies through cultivation, Auricularia auricula, Collybia velutipes, Coriolus versicolor, G. capense, G. japonicum, G. lucidum, Hericium erinaceus, Pleurotus cornucopiae, P. ostreatus, P. sapidus, Poria cocos, Schizophyllum commune and Volvariella volvacea were able to retain their inherent nature, while only Armillariella tabescens, Lentinus edodes and Tremella fuciformis were found to lose the ability to form fruit bodies. The mineral oil seal is much simpler and it does not require special equipment. It is a suitable method for preserving Basidiomycetes cultures in large quantities.

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Accession: 000819676

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