Anther culture of wheat (Triticum aestivum L.) F1's and their reciprocal crosses
Bullock, W.P.; Baenziger, P.S.; Schaeffer, G.W.; Bottino, P.J.
Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 62(2): 155-159
ISSN/ISBN: 0040-5752 PMID: 24270565 DOI: 10.1007/bf00293350
Anthers from 3 sets of wheat (T. aestivum L. em. Thell) F1's and their reciprocal crosses, made between parental lines differing greatly in their ability to produce microspore derived callus, were cultured on the Chinese potato medium in a study to define more clearly the role of nuclear or cytoplasmic factors within T. aestivum in transferring the ability to undergo in vitro androgenesis, and to briefly review the gametic representation and disease screening potential of the resulting polyhaploid wheat plants. The microspore derived calli values from F1 were slightly less than the midparental value. Statistical analysis indicated that the ability of each F1 to produce callus either did not significantly differ from that of the respective parental line having the highest androgenic yield or it exceeded its respective parental line having the lowest yield. No differences were noted between the members of each pair of reciprocal crosses. The transfer of in vitro androgenic ability to F1 hybrids is not dependent upon the maternal cytoplasm source. Polyhaploid plants, carrying the Pm 3 a powdery mildew [Erysiphe graminis tritici] resistance gene, expressed resistance to culture 4a of powdery mildew.